2004. 5. 19
MicroRNAs (miRNAs) are transcribed as long primary transcripts (pri-miRNAs) which are then matured through at least two sequential events: the nuclear processing of the pri-miRNAs into stem-loop precursors of ~70-nt (pre-miRNAs) and the cytoplasmic processing of pre-miRNAs into mature miRNAs of ~22-nt. Dicer, a member of the Ribonuclease III (RNase III) superfamily of bidentate nucleases, mediates the latter step, whereas the processing enzyme for the former step remained unknown.
We recently identified another RNase III type protein, Drosha, as the core nuclease that executes the initiation step of miRNA processing in the nucleus. Further analysis indicated that Drosha predetermines the sequences of mature miRNA by precisely cropping pri-miRNA. The mechanism for the specific selection of cleavage sites will be discussed.
Although transcription is another key step in miRNA biogenesis, little is known about the transcriptional machinery and gene structure. We have characterized the structure of primary transcript of a miRNA and determined the promoter. Our data provide a critical insight into the transcriptional regulation of miRNA biogenesis.
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2001-2004 ¼¿ï´ëÇÐ±³ BK21»ý¸í°úÇÐÀÎ·Â¾ç¼º»ç¾÷´Ü °è¾à±³¼ö
1999-2001 University of Pennsylvania, postdoctoral fellow
1994-1998 Oxford University »ýÈÇÐ°ú, ¹Ú»ç
1992-1994 ¼¿ï´ëÇÐ±³ ¹Ì»ý¹°ÇÐ°ú, ¼®»ç
1988-1992 ¼¿ï´ëÇÐ±³ ¹Ì»ý¹°ÇÐ°ú, ÇÐ»ç