Application of Micro & Nanobeads for Bio Application
2004. 5. 17
After human genome project is over, the demand for developing a rapid and effective method for the identification of a target protein and its drug-like chemical ligand is increasing. For this, the high throughput screening (HTS) systems using micron-sized beads, which can be tagged with fluorescent dyes or quantum dots have been developed. The micro-beads can also be encoded by nanoparticles with tagging chemicals, which are subjected to optical or surface enhanced Raman scattering (SERS) detection. The chemical libraries can be generated on the micro beads by split-and-pool method and the binding events with a target protein can be analyzed by spectroscopic methods.
To utilize these HTS systems in screening the chemical libraries and a target protein effectively, the polymeric beads should be compatible with chemical and biological system. So far, we have developed various kinds of micro-beads. Especially, we have proposed the concept of the core-shell type beads, which has low nonspecific adsorption of protein that has been serious problem in bio-assay and protein purification.
Recently, we have been successful in the application of our micro-beads to the micro system for the detection of a target protein such as HCV polymerase and DNA binding protein. For efficient manipulation of the micro beads in the micro-system, magnetic beads were prepared by post-magnetization method. Additionally, our recent works on the development of micro-beads, which are suitable for peptide and DNA synthesis or peptide ligation will be presented and discussed.
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