Genome-wide analysis on post-transcriptional RNA regulation

Woong-Yang Park

College of Medicine, Seoul National University

2007. 3. 21

301-421, 11:00 AM

 

One-fourth of all functionally annotated human genes are coding nucleic-acid binding proteins. Genome-wide analysis on their dimensions has not yet been investigated against most of these proteins. Especially post-transcriptional regulations of RNA like splicing, editing, nonsense mRNA decay are now proposed to be an important mechanism for the neuronal function, but the limited information RNA-binding proteins restricts a comprehensive understanding of these protein functions. UV cross-linking is a method typically used to determine the position of direct interactions between proteins and nucleic acids. Cross-linking and immunoprecipitation assay can exploit the covalent protein-nucleic acid cross-linking to stringently purify a specific protein-RNA complex using immunoprecipitation followed by SDS-PAGE separation. RNA tags from RNA binding proteins can be analyzed by high throughput genotyping and sequence analysis, which will enable us to understand their functions in detail.

 

This page is maintained by Ji-seon Yoo (jsyoo@bi.snu.ac.kr).
Last update: June 15, 2007