Regulation of Coding and ncRNAs through multiple AGO-siRNA Pathways
Whitehead Institute at MIT
January 14, 2011 1:30 PM
Endogenous short interference RNAs (siRNAs) function as regulators in defense mechanisms such as the viral protection and suppression of transposons as well as developmental processes such as germline development in animals. C.elegans genomes transcribe various classes of endogenous siRNAs loaded into multiple argonautes (AGOs) that direct transcriptional and post-transcriptional regulations. Analyzing mRNA cleavage tags and small RNAs in wild type and germline-deficient animals first reveals not only cleavage targets of endogenous siRNAs, but also multiple cleavage pathways of specific endogenous siRNA groups. Investigating WAGO-1 and CSR-1 specific 22G RNA targets on microarray data of wilde type and drh-3 mutant elucidates in vivo catalytic cleavage activity of CSR-1 that directs down-regulation of its targets.
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