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Regulation of Coding and ncRNAs through multiple AGO-siRNA Pathways Jin-Woo Nam Whitehead Institute at MIT January 14, 2011 1:30 PM 302-408 Endogenous short interference RNAs (siRNAs) function as
regulators in defense mechanisms such as the viral protection and suppression
of transposons as well as developmental processes such as germline
development in animals. C.elegans genomes transcribe various classes of
endogenous siRNAs loaded into multiple argonautes (AGOs) that direct
transcriptional and post-transcriptional regulations. Analyzing mRNA cleavage
tags and small RNAs in wild type and germline-deficient animals first reveals
not only cleavage targets of endogenous siRNAs, but also multiple cleavage
pathways of specific endogenous siRNA groups. Investigating WAGO-1 and CSR-1
specific 22G RNA targets on microarray data of wilde type and drh-3 mutant
elucidates in vivo catalytic cleavage activity of CSR-1 that directs
down-regulation of its targets. This page is maintained by Jin-young Chun (jychun@bi.snu.ac.kr). |